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APAF offers high abundant protein removal services to customers for “deep drilling” of proteome. If the low abundant proteins in a sample are of interests, it is essential to remove the high abundant proteins first to facilitate concentrating more of low abundant proteins for downstream proteomics analysis. We provide high abundant protein removal services for serum, plasma and cerebral spinal fluid (CSF) samples of humans and rodents (rat and mouse).
APAF is facilitated with two high abundant protein removal systems for human samples using immunodepletion technologies.
1. Simultaneously removes top 12 high abundant proteins (approximately 95% of total protein mass of plasma) such as Albumin, IgG, Fibrinogen, Transferrin, IgA, IgM, Haptoglobin, alpha 2-Macroglobulin, alpha 1-Acid Glycoprotein, alpha 1-Antitrypsin and HDL (Apo A-I & Apo A-II).
2. Simultaneously removes 6 high abundant proteins (approximately 85-90% of total protein mass of plasma) such as Albumin, IgG, alpha 1-Antitrypsin, IgA, Transferrin, and Haptoglobin.
Selective immunodepletion provides an enriched pool of low abundant proteins for downstream proteomics analysis. Removal of high-abundant proteins enables improved resolution and dynamic range for one-dimensional (1-D), two-dimensional (2-D) gel electrophoresis and liquid chromatography mass spectrometry (LC MS). The collected flow-through fractions may need to be concentrated dependent upon the downstream applications.
Simultaneously removes 7 high abundant proteins such as Albumin, IgG, Fibrinogen, Transferrin, IgM, Haptoglobin, alpha 1-Antitrypsin. Specific removal of 7 high abundant proteins depletes more than 80% of total protein mass from mouse or rat serum or plasma. The low-abundant proteins in the flow-through fractions can be studied for downstream proteomic analysis.
For further details or advice on this APAF service, please contact us at apafinfo@proteome.org.au. |