Phosphoproteomics - NEW service!
Protein phosphorylation is a ubiquitous and very important post-translational modification (PTM), which affects an estimated one third of all proteins. Many critical events involved in cellular response are mediated by phosphorylation and dephosphorylation, such as regulation of enzymatic activity, protein conformational change, protein-protein interaction, and cellular localization. Abnormal phosphorylation often leads to severe diseases.
Phosphoproteomics refers to the global analysis of the phosphate modified proteome. Typically phosphorylated amino acids include serine, threonine, and tyrosine and at APAF we have established methods to enrich and analyse peptides containing these modified residues. For phospho-serine/threonine peptides we use a combination of ion exchange chromatography and titanium dioxide enrichment prior to LC/MS. For phosphotyrosine containing peptides immunoaffinity capture is used.
APAF is your one-stop PTM shop. We have the equipment required.