Multiple reaction monitoring mass spectometry (MRM-MS) is a highly selective and sensitive method used to quantitate protein/peptide abundances in complex biological samples for targeted quantitation of proteins. MRM-MS is commonly utilised for small molecule analysis and is now developing as a mainstream technology for proteomics. Scheduled MRM-MS assays are compatible with multiplexing, enabling the quantitation for tens-to-hundreds of analytes in a single MS run.
For absolute quantitative analysis, synthetic peptides containing a stable isotopically labelled amino acid are used as standards. This enables the performance of each assay to be assessed for limits of detection/quantitation and linearity. MRM-MS assays can also be used for relative quantitation of analytes across runs.
Absolute Quantitation - SRM/MRM-MS
At APAF we have developed over 200 MRM assays for human plasma and cell lysate proteins. With MRM, absolute quantitation is available however there are many pitfalls to the unwary that can compromise accuracy. A major benefit of MRM is the ability to multiplex many assays, allowing fast and accurate quantitation within the MS analysis. At APAF, if we can observe the peptide by MS we can develop an MRM assay for you.
Our Expertise Includes:
- Leading edge instrumentation and software
- Expert knowledge in MRM assay signal optimisation
- Demonstrated low CV assays
- Extensive publication record
- Patent applications on the selection of MRM transition ions
DOES YOUR PROTEIN QUANTITATION NEED A BOOST? LOOK TO APAF FOR ASSAY DEVELOPMENT AND ANALYSIS.