Australian Proteome Analysis Facility  
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Recent Addition

New catalogue and services.

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APAF has recently added Difference Gel Electrophoresis (DIGE) to our available proteomics services. DIGE allows researchers to focus only on proteins of relevance whilst simultaneously removing gel to gel variation by the incorporation of an internal standard for every gel spot.
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Amino Acid Analysis

Amino Acid Analysis is one of the best quantitative methods in protein chemistry and used by many laboratories for peptide/protein characterization. Whilst a well established technique in protein chemistry, complications can come from a variety of sources including

  • variability in the inherent lability of amino acids to hydrolysis, oxidation and modification
  • variability in instrument performance and hydrolysis/derivatisation conditions
  • background or sample contamination
  • data reduction methods
  • work habits

APAF has refined Amino Acid Analysis methodologies ensuring accurate and reliable results from a broad range of sample types including

  • food stuffs
  • traditional medicines
  • recombinant protein sources
  • plant materials

For further details or advice on this APAF service please contact us at aaa@proteome.org.au.

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