iTRAQ^TM

iTRAQ^TM (isobaric tag for relative and absolute quantitation) is a chemical derivatisation technique used to identify, compare and quantify proteins from multiple samples in one single experiment by Mass Spectrometry.

iTRAQ^TM reagents are supplied by AB Sciex as an 8-plex kit. However, if more than eight samples are to be analysed, a common reference sample should also be included to allow comparisons in all subsequent iTRAQ^TM experiments.

Benefits:

• Multiplexing saves you time
• Cheaper than label free if paying for contract service
• Within spectra relative quantitation means no missing values
• APAF has 5 years experience using iTRAQ^TM-MS

Comparative Proteomics: iTRAQ^TM

A combination of 2DLC and electrospray LC/MS requires expertise in sample preparation, chromatography and MS to ensure reproducible results. This can be a difficult combination of techniques to master for many researchers.

Our Expertise Includes:

1. International benchmarking rank us in the top tier of facilities
2. Published articles with leading pharmaceutical companies and renowned researchers
3. Proficiency to develop new workflow to give scientists more options to use iTRAQ^TM for their experiments
4. Pathway analysis - included as a bonus for model organisms

LET APAF TAKE THE UNCERTAINTY OUT OF YOUR RESEARCH. TALK TO US ABOUT AN 8-PLEX iTRAQ^TM EXPERIMENT.

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Last updated 29 Oct 2010