Services	Number of samples/Comment	Fees per sample (Excl. GST)
MALDI-TOF (PMF) for protein identification 1	1-12	$180
	13-30	$155
	30-96 (Full Plate)	$4800 per plate
MALDI-TOF/TOF (PMF+MS/MS) for protein identification 1	1-12	$235
	13-30	$215
	30-96 (Full Plate)	$8640 per plate
1D nanoLC ESI MS/MS for protein identification 2	1-5	$450
	6+	$400
Mass Analysis of Intact Protein	1-5	$190
	6+	$170
Static nanospray for peptide mass analysis and MS/MS	1+	$180
De Novo peptide sequencing 3	1-2 peptides from the same sample	$150
	More than 2 peptides from the same sample	$150 plus $75 per extra peptide
Desalting with porous R2 beads micro column 4	1+	$50
Reduction and alkylation 5	1+	$150
Special sample treatment 6	Price on application 7
2D LC ESI MS/MS	Price on application 7
Phosphopeptide analysis	Price on application 7
Protein quantitation using iTRAQ	Price on application 7
Oligosaccharide analysis	Price on application 7
Amino Acid Analysis	High Sensitivity AAA Duplicates (gas phase Analysis)	$110
	Free AAA Duplicates (no hydrolysis)	$110
	Quantitative AAA Duplicates (for food products - liquid hydrolysis)	$130
	Tryptophan Determination Duplicates (base hydrolysis) 8	$150
	Cysteine Determination Duplicates (performic acid oxidation) 8	$160
	Sample desalting	$60
	Special Amino Acids	Price on application 7
N-Terminal Sequencing	Set Up	$170
	Edman sequencing fee per amino acid 9	$30
	Cysteine reduction/alkylation	$150
	Elution of protein from gel prior to analysis	$60
	No N-terminal sequence obtained	$290
HPLC	Price on application 7
High Abundance Protein Removal	MARS Column, top 7 abundant proteins	$100
	Genway Column, top 12 abundant proteins	$150
Protein Quantitation Determination	eg. Bradford Assay	$50
1D Gel		$100
Mini 2D gel (11cm) (pH range: 3-10; 4-7; 6-11)		$180
Blot from mini gel (Nitrocellulose or PVDF)	Price on application 7
Large format 2D gel (17cm) (pH range: 3-10; 4-7; 6-11)		$480
Blot from large format gel (Nitrocellulose or PVDF)	Price on application 7
2D Gel Image Analysis		$150 per hour
Difference Gel Electrophoresis (DIGE)	Price on application 7
Luminex - Cytokine Analysis	Price on application 7
Bioactive Discovery	Price on application 7
Research Hotel	Price on application 7
Sugar Analysis (Dionex)	Price on application 7
Protein Production	Price on application 7
Customs Clearance	For International Customers Only	$60

Notes:
1. MALDI-TOF (PMF) and MALDI-TOF/TOF (PMF + MS/MS) services include protein digestion, desalting and peptide concentration using a commercial C18 micro column, MS data acquisition, Mascot database searching and a standard report. Prices for samples that result in one or more database matches are the same as the price for samples where a match is not found.

2. 1D nanoLC ESI MS/MS services include protein digestion, reversed phase nanoLC and MS data acquisition, Mascot database searching and a standard report. Prices for samples that result in one or more database matches are the same as the price for samples where a match is not found.

3. De Novo peptide sequencing is undertaken an additional data analysis service based on the data acquired from MALDI-TOF/TOF or ESI MS/MS when there is no matches from automated database searches.

4. Desalting with porous R2 beads micro column is a service using self packed porous R2 micro column to desalt and concentrate proteins and peptides. This is recommended if salt needs to be removed from solution for intact protein mass analysis. It is also recommended for 2D gel faint spot MALDI MS analysis. 

5. Reduction and alkylation of samples prior to shipping is recommended. Should APAF need to perform this work, the cost will be passed on to the customer. Sample reduction and alkylation is recommended when the following services are requested: MALDI-TOF (PMF), MALDI-TOF/TOF (PMF + MS/MS) and LC ESI MS/MS for protein identification.  Note: Spots submitted from 2-D gels are usually alkylated during the process of 2-DE and therefore do not require any subsequent alkylation steps.

6. Special sample treatment includes other services such as extracting proteins from samples, depleting high abundant proteins, 1D SDS or 2D gel electrophoresis for protein separation, HPLC for protein separation and image analysis.

7. Please contact APAF to discuss the scope of the services required and associated fees.

8. Other amino acids available upon request (e.g. hydroxyproline, taurine).

9. A fee based upon set up costs and per residue sequenced is charged. Approximately 10 residues are normally sequenced to get an identification.