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Prices for Mass Spectrometry
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Service Type
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Service Details
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Price commercial
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4800 MALDI-TOF/TOF (PMF+MS/MS) for Protein Identification; 1 - 96 samples 1
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Include trypsin digestion, zip tipping clean up and concentrating samples on AnchorChip plate, MS data acqusition, database search and report
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$170/sample, maximum $6300
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ID nanoLC ESI MS/MS for Protein Identification; 1 - 5 samples 2
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Trypsin digestion, reverse phase nanoLC ESI MS data acqusition, database search and report
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$350/sample
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ID nanoLC ESI MS/MS for Protein Identification; 6 + samples 2
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Trypsin digestion, reverse phase nanoLC ESI MS data acqusition, database search and report
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$330/sample
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Mass Analysis of Intact Protein by MALDI or direct infusion ESI MS
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MS data acqusition and report
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$220 for the first sample, $125/sample for second or more samples
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De Novo Peptide Sequencing 3
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Data analysis only. Sample prep and MS data acqusition extra.
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$75/peptide
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Intact Mass of Protein after removal of N-Linked glycans by MALDI MS
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Including removal of N-linked glycans, sample desalting, MS data acqusition and report
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$550/sample
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MALDI-TOF/TOF protein IDs for Glycoprotein from gel spot or membrane
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Including removal of glycans, trypsin digest of the protein, MALDI TOF/TOF data acquisition, database search and report
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$390/sample
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Oligosaccharide Analysis
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Including spot bloting glycoproteins on membrane, extract glycans, desalt, 1D LC ESI MS data acqusition and data analysis of the glycans
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Price on Application 5
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Intact Mass including Glycans by RP (C4 or C5) LC ESI MS
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LC MS setup for the glycoprotein analysis, test run, data acqusition and report
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Price on Application 5
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ESI MS of peptides
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Direct sample infusion ESI MS data acquisition
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$80/sample
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LC ESI MS of peptides
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Reverse phase HPLC ESI MS for peptides or organic compounds
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$120/sample
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High resolution high mass accuracy ESI MS analysis of purified peptides
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Direct sample infusion ESI MS data acquisition, intermal calibration, elemental composition match report
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$100/sample
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Hourly rate for MS Data analysis
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Data analsysis
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$150
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LC-MS Hourly Rate
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Instrument run time
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$150
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Special Sample Treatment 4
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Such as protein extraction, SCX to remove detergent, buffer exchange, acetone precipitation
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Price on Application 5
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2D LC ESI MS/MS
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Include trypsin digestion, offline SCX fractionation, reverse phase nanoLC 2D nanoLC ESI MS/MS data acqusition, database search and report
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Price on Application 5
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Phosphopeptide Analysis
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Trypsin digestion, phosphopeptide enrichment, reverse phase nanoLC ESI MS/MS analysis, database search and report
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Price on Application 5
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Protein Quantitation using iTRAQ
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Reduction/alkylation, trypsin digestion, iTRAQ labeling, 2D LC ESI MS/MS, data processing using and report
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Price on Application 5
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MRM/SRM Assay Development
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Method development for targeted proteins or peptides
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Price on Application 5
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MRM/SRM Sample Analysis
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Using the developed method to acquire data for the targeted proteins or peptides, data analysis, report
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Price on Application 5
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Prices for Protein Chemistry
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Service Type
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Service Details
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Price commercial
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Edman Sequencing set-up fee
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Edman sequencing set-up per sample
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$190
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Edman Sequencing per Amino Acid
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Edman sequencing of each amino acid residue cycle
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$33
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Cys Reduction/Alkylation
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Derivatisation of cysteine residues to enable identification + sample desalting
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$150
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Elution from Gel
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Passive elution of intact protein from gel samples for edman sequencing
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$65
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Sample Desalting
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Removal of interfering buffers/salts, etc prior to sequencing
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$65
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High Sensitivity AAA
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For the analysis of amino acids in µg quantities of purified protein. Uses gas phase acid hydrolysis. Duplicate analysis.
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$110
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Free AAA Standard Amino Acids
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Analysis of free amino acids in culture media, physiological samples, etc. No hydrolysis employed. Duplicate analysis.
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$110
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| Free AAA physiological fluids |
Upto 37 Amino Acids. Duplicate analysis |
$150 |
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Quantitative AAA
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For the analysis of amino acids in unpurified, complex samples such as food products. Employs liquid phase acid hydrolysis to extract protein-bound amino acids. Duplicate analysis.
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$130
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Tryptophan Determination 6
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Analysis of tryptophan in proteins only. Employs base hydrolysis conditions (Trp is destroyed by the acid hydrolysis conditions of Quantitaive AAA). Duplicate analysis.
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$150
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Cysteine Determination 6
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For the analysis of Cysteine/Cystine in proteins (Cys is partially destroyed by acid hydrolysis conditions of AAA). Employs performic acid oxidation to convert Cys to more stable cysteic acid. Duplicate analysis.
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$160
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Special amino acid analysis (eg. hydroxyproline, hydroxylysine, taurine, asparagine, glutamine, ornithine, aminosugars, etc)
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For the analysis of less common amino acids please enquire, including an enzymatic hydolysis procedure for acid labile amino acids such as glutamine and asparagine.
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Price on application 5
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Sample Desalting
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Sample preparation - removal of salt, buffers, etc prior to AAA
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$65
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Reversed Phase (RP) HPLC
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RP-HPLC analysis of proteins, peptides and small molecules. Suitable for the quantitation and small scale purification.
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Price on application 5
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Ion Exchange Chromatography (IEX)
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Anion or cation exchange separation of proteins and peptides with fraction collection
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Price on application 5
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Size Exclusion Chromatography (SEC)
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Separation of proteins and peptides based on size. Three MW range columns are available for high MW proteins, low MW proteins and peptide SEC
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Price on application 5
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Peptide Mapping
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RP-HPLC of tryptic digest after Cys reduction/alkylation. Peptide identication by edman sequencing or MS can also be undertaken.
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Price on application 5
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2D LC MS/MS
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Trypsin digestion, offline strong cation exchange (SCX) fractionation, RPnanoLC ESI MS/MS
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Price on application 5
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Disulfide mapping
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Protein digestion with a suitable enzyme followed by RP-HPLC and peptide ID by edman and/or MS analysis.
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Price on application 5
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High Abundance Protein Removal - top 14 (human)
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Affinity chromatography depletion of top 14 abundant proteins form serum, plasma, urine, etc.
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Price on application 5
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Sugar/Carbohydrate analysis using Dionex HPLC
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Monosaccharide and carbohydrate analysis by Dionex LC anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD)
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Price on application 5
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Prices for Array Technologies
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Service Type
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Service Details
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Price commercial
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High Abundance Protein Removal serum- top 6 (human and/or rodent)
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Collect flow through proteins (low abundant), concentrate by precipitation, protein pellete dissolve in a buffer
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Price on application 5
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High Abundance Protein Removal from serum- top 7 (human)
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Collect flow through proteins (low abundant), concentrate by precipitation, protein pellete dissolve in a buffer
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Price on application 5
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High Abundance Protein Removal serum- top 14 (human)
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Collect flow through proteins (low abundant), concentrate by precipitation, protein pellete dissolve in a buffer
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Price on application 5
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Sample preparation from tissue for 2-DE
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Extraction of proteins, desalting, quantitation by Bradford assay and validation on 1-D gel
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$150 for the first sample; $50/sample for second or more samples
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Sample preparation from microorganisms for 2-DE
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Extraction of proteins, desalting, quantitation by Bradford assay and validation on 1-D gel
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$150 for the first sample; $50/sample for second or more samples
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Sample preparation from plants for 2-DE
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Extraction of proteins, desalting, quantitation by Bradford assay and validation on 1-D gel
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$200 for the first sample; $75/sample for second or more samples
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Sample preparation from human body fluids (+/- depletion)
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Concentration, desalting, quantitation by Bradford assay and validation on 1-D gel
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$100 for the first sample; $50/sample for second or more samples
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Protein Quantitation
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Using Bradford or any other available method
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$150 per batch (1 to 72 wells per batch)
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1D gel up to 18 wells - Fluorescent stained
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Run gel, stain with SYPRO Ruby, Flamingo Pink, Lava Purple, etc., Image capture with a Typhoon Scanner
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$150 per gel
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1D gel up to 18 wells - Coomassie stained
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Run gel, stain with Coomassie, image capture with a flatbed scanner
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$90 per gel
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11cm mini 2-D gel - Fluorescent stained
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Run gel, any pH range, stain with SYPRO Ruby or Flamingo Pink, or Lava Purple, etc., image capture with a Typhoon scanner
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$180 per gel
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11cm mini 2-D gel - Coomassie stained
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Run gel, any pH range, gel stain with Coomassie, image capture with a flatbed scanner
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$120 per gel
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17 or 18 cm format 2-D gel - Fluorescent stained
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Run gel, any pH range, gel stain with SYPRO Ruby or Flamingo Pink, or Lava Purple, etc., image capture with a Typhoon scanner
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$350 per gel
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17 or 18 cm format 2-D gel - Coomassie stained
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Run gel, any pH range, gel stain with Coomassie, image capture with a flatbed scanner
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$200 per gel
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24 cm format 2-D gel - Fluorescent stained
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Run gel, any pH range, gel stain with SYPRO Ruby or Flamingo Pink, or Lava Purple, etc., image capture with a Typhoon scanner
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$400 per gel
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24 cm format 2-D gel - Coomassie stained
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Run gel, any pH range, gel stain with Coomassie, image capture with a flatbed scanner
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$250 per gel
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Difference Gel Electrophoresis (DIGE) saturation labelling
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Sample labelling with Cy3 and Cy5, run gel, image capture with a Typhoon scanner
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$600 per gel (up to 4 gels)
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Difference Gel Electrophoresis (DIGE) minimal labelling
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Sample labelling with Cy2, Cy3 and Cy5, run gel, image capture with a Typhoon scanner
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$1,200 per gel (up to 4 gels)
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Gel Image Analysis/Differential display analysis
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Image analysis using Progenesis software, interpretation of results and report writing
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$100 per hour or ask for a quote for bulk
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Transfer proteins from mini gel (1-D or 2-D) to membrane
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Run gel, transfer proteins on a membrane, membrane stain with Coomassie, image capture with a flatbed scanner (no Western analysis)
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$260 per blot
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Transfer proteins from large formate 2-D gel (18 cm) to membrane
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Run gel, transfer proteins on a membrane, membrane stain with Coomassie, image capture with a flatbed scanner (no Western analysis)
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$350 per blot
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Western analysis (1-D gel, up to 10 wells) - using client supplied primary antibody
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Run gel, transfer proteins to membrane, probe with antibodies, ECL detection and image capture with a Fuji LAS 3000 CCD camera
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$300 per Western blot
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Western analysis (1-D gel, up to 18 wells) - using client supplied primary antibody
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Run gel, transfer proteins to membrane, probe with antibodies, ECL detection and image capture with a Fuji LAS 3000 CCD camera
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$325 per Western blot
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Western analysis (11 cm 2-D gel) - using client supplied primary antibody
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Run gel, transfer proteins to membrane, probe with antibodies, ECL detection and image capture with a Fuji LAS 3000 CCD camera
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$360 per Western blot
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Western analysis (17 cm 2-D gel) - using client supplied primary antibody
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Run gel, transfer proteins to membrane, probe with antibodies, ECL detection and image capture with a Fuji LAS 3000 CCD camera
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$450 per Western blot
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BioPlex/Luminex - instrument (daily access)
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Instrument calibration and/or validation, and operation (Robot and plate reading not included)
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$150 per day
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BioPlex/Luminex - multiplex analysis DIY
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Using an EpMotion robot for assay or plate reading with BioPlex/Luminex, etc.
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$150 per hour
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BioPlex/Luminex - multiplex analysis
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Instrument calibration and/or validation, conducting assay using an EpMotion robot, plate reading and report writing
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$800 per plate + kit cost
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Instrument access DIY
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Access to any instruments (limitations apply)
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$150 hour
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