Prices for Mass Spectrometry
Service Type	Service Details	Price commercial
4800 MALDI-TOF/TOF (PMF+MS/MS)  for Protein Identification; 1 - 96 samples 1	Include trypsin digestion, zip tipping clean up and concentrating samples on AnchorChip plate, MS data acqusition, database search and report	$170/sample, maximum $6300
ID nanoLC ESI MS/MS for Protein Identification; 1 - 5 samples 2	Trypsin digestion, reverse phase nanoLC ESI MS data acqusition, database search and report	$450/sample
ID nanoLC ESI MS/MS for Protein Identification; 6 + samples 2	Trypsin digestion, reverse phase nanoLC ESI MS data acqusition, database search and report	$400/sample
Mass Analysis of Intact Protein by MALDI or direct infusion ESI MS	MS data acqusition and report	$220 for the first sample, $125/sample for second or more samples
De Novo Peptide Sequencing 3	Data analysis only. Sample prep and MS data acqusition extra.	$75/peptide
Intact Mass of Protein after removal of N-Linked glycans by MALDI MS	Including removal of N-linked glycans, sample desalting, MS data acqusition and report	$550/sample
MALDI-TOF/TOF protein IDs for Glycoprotein from gel spot or membrane	Including removal of glycans, trypsin digest of the protein, MALDI TOF/TOF data acquisition, database search and report	$390/sample
Oligosaccharide Analysis	Including spot bloting glycoproteins on membrane, extract glycans, desalt, 1D LC ESI MS data acqusition and data analysis of the glycans	Price on Application 5
Intact Mass including Glycans by RP (C4 or C5) LC ESI MS	LC MS setup for the glycoprotein analysis, test run, data acqusition and report	Price on Application 5
ESI MS of peptides	Direct sample infusion ESI MS data acquisition	$80/sample
LC ESI MS of peptides	Reverse phase HPLC ESI MS for peptides or organic compounds	$120/sample
High resolution high mass accuracy ESI MS analysis of purified peptides	Direct sample infusion ESI MS data acquisition, intermal calibration, elemental composition match report	$100/sample
Hourly rate for MS Data analysis	Data analsysis	$150
LC-MS Hourly Rate	Instrument run time	$150
Special Sample Treatment 4	Such as protein extraction, SCX to remove detergent, buffer exchange, acetone precipitation	Price on Application 5
2D LC ESI MS/MS	Include trypsin digestion, offline SCX fractionation, reverse phase nanoLC  2D nanoLC ESI MS/MS data acqusition, database search and report	Price on Application 5
Phosphopeptide Analysis	Trypsin digestion, phosphopeptide enrichment, reverse phase nanoLC ESI MS/MS analysis, database search and report	Price on Application 5
Protein Quantitation using iTRAQ	Reduction/alkylation, trypsin digestion, iTRAQ labeling, 2D LC ESI MS/MS, data processing using and report	Price on Application 5
MRM/SRM Assay Development	Method development for targeted proteins or peptides	Price on Application 5
MRM/SRM Sample Analysis	Using the developed method to acquire data for the targeted proteins or peptides, data analysis, report	Price on Application 5
Prices for Protein Chemistry
Service Type	Service Details	Price commercial
Edman Sequencing set-up fee	Edman sequencing set-up per sample	$170
Edman Sequencing per Amino Acid	Edman sequencing of each amino acid residue cycle	$30
Cys Reduction/Alkylation	Derivatisation of cysteine residues to enable identification + sample desalting	$150
Elution from Gel	Passive elution of intact protein from gel samples for edman sequencing	$60
Sample Desalting	Removal of interfering buffers/salts, etc prior to sequencing	$60
High Sensitivity AAA	For the analysis of amino acids in µg quantities of purified protein. Uses gas phase acid hydrolysis. Duplicate analysis.	$110
Free AAA	Analysis of free amino acids in culture media, physiological samples, etc. No hydrolysis employed. Duplicate analysis.	$110
Quantitative AAA	For the analysis of amino acids in unpurified, complex samples such as food products. Employs liquid phase acid hydrolysis to extract protein-bound amino acids.  Duplicate analysis.	$130
Tryptophan Determination 6	Analysis of tryptophan in proteins only.  Employs base hydrolysis conditions (Trp is destroyed by the acid hydrolysis conditions of Quantitaive AAA). Duplicate analysis.	$150
Cysteine Determination 6	For the analysis of Cysteine/Cystine in proteins (Cys is partially destroyed by acid hydrolysis conditions of AAA). Employs performic acid oxidation to convert Cys to more stable cysteic acid. Duplicate analysis.	$160
Special amino acid analysis (eg. hydroxyproline, hydroxylysine, taurine, asparagine, glutamine, ornithine, aminosugars, etc)	For the analysis of less common amino acids please enquire, including an enzymatic hydolysis procedure for acid labile amino acids such as glutamine and asparagine.	Price on application 5
Sample Desalting	Sample preparation - removal of salt, buffers, etc prior to AAA	$60
Reversed Phase (RP) HPLC	RP-HPLC analysis of proteins, peptides and small molecules. Suitable for the quantitation and small scale purification.	Price on application 5
Ion Exchange Chromatography (IEX)	Anion or cation exchange separation of proteins and peptides with fraction collection	Price on application 5
Size Exclusion Chromatography (SEC)	Separation of proteins and peptides based on size. Three MW range columns are available for high MW proteins, low MW proteins and peptide SEC	Price on application 5
Peptide Mapping	RP-HPLC of tryptic digest after Cys reduction/alkylation. Peptide identication by edman sequencing or MS can also be undertaken.	Price on application 5
2D LC MS/MS	Trypsin digestion, offline strong cation exchange (SCX) fractionation, RPnanoLC ESI MS/MS	Price on application 5
Disulfide mapping	Protein digestion with a suitable enzyme followed by RP-HPLC and peptide ID by edman and/or MS analysis.	Price on application 5
High Abundance Protein Removal - top 14 (human)	Affinity chromatography depletion of top 14 abundant proteins form serum, plasma, urine, etc.	Price on application 5
Sugar/Carbohydrate analysis using Dionex HPLC	Monosaccharide and carbohydrate analysis by Dionex LC anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD)	Price on application 5
Prices for Array Technologies
Service Type	Service Details	Price commercial
High Abundance Protein Removal serum- top 6 (human and/or rodent)	Collect flow through proteins (low abundant), concentrate by precipitation, protein pellete dissolve in a buffer	Price on application 5
High Abundance Protein Removal from serum- top 7 (human)	Collect flow through proteins (low abundant), concentrate by precipitation, protein pellete dissolve in a buffer	Price on application 5
High Abundance Protein Removal serum- top 14 (human)	Collect flow through proteins (low abundant), concentrate by precipitation, protein pellete dissolve in a buffer	Price on application 5
Sample preparation from tissue for 2-DE	Extraction of proteins, desalting, quantitation by Bradford assay and validation on 1-D gel	$150 for the first sample; $50/sample for second or more samples
Sample preparation from microorganisms for 2-DE	Extraction of proteins, desalting, quantitation by Bradford assay and validation on 1-D gel	$150 for the first sample; $50/sample for second or more samples
Sample preparation from plants for 2-DE	Extraction of proteins, desalting, quantitation by Bradford assay and validation on 1-D gel	$200 for the first sample; $75/sample for second or more samples
Sample preparation from human body fluids (+/- depletion)	Concentration, desalting, quantitation by Bradford assay and validation on 1-D gel	$100 for the first sample; $50/sample for second or more samples
Protein Quantitation	Using Bradford or any other available method	$150 per batch (1 to 72 wells per batch)
1D gel up to 18 wells - Fluorescent stained	Run gel, stain with SYPRO Ruby, Flamingo Pink, Lava Purple, etc., Image capture with a Typhoon Scanner	$150 per gel
1D gel up to 18 wells - Coomassie stained	Run gel, stain with Coomassie, image capture with a flatbed scanner	$90 per gel
11cm mini 2-D gel - Fluorescent stained	Run gel, any pH range, stain with SYPRO Ruby or Flamingo Pink, or Lava Purple, etc., image capture with a Typhoon scanner	$180 per gel
11cm mini 2-D gel - Coomassie stained	Run gel, any pH range, gel stain with Coomassie, image capture with a flatbed scanner	$120 per gel
17 or 18 cm format 2-D gel - Fluorescent stained	Run gel, any pH range, gel stain with SYPRO Ruby or Flamingo Pink, or Lava Purple, etc., image capture with a Typhoon scanner	$350 per gel
17 or 18 cm format 2-D gel - Coomassie stained	Run gel, any pH range, gel stain with Coomassie, image capture with a flatbed scanner	$200 per gel
24 cm format 2-D gel - Fluorescent stained	Run gel, any pH range, gel stain with SYPRO Ruby or Flamingo Pink, or Lava Purple, etc., image capture with a Typhoon scanner	$400 per gel
24 cm format 2-D gel - Coomassie stained	Run gel, any pH range, gel stain with Coomassie, image capture with a flatbed scanner	$250 per gel
Difference Gel Electrophoresis (DIGE) saturation labelling	Sample labelling with Cy3 and Cy5, run gel, image capture with a Typhoon scanner	$600 per gel (up to 4 gels)
Difference Gel Electrophoresis (DIGE) minimal labelling	Sample labelling with Cy2, Cy3 and Cy5, run gel, image capture with a Typhoon scanner	$1,200 per gel (up to 4 gels)
Gel Image Analysis/Differential display analysis	Image analysis using Progenesis software, interpretation of results and report writing	$100 per hour or ask for a quote for bulk
Transfer proteins from mini gel (1-D or 2-D) to membrane	Run gel, transfer proteins on a membrane, membrane stain with Coomassie, image capture with a flatbed scanner (no Western analysis)	$260 per blot
Transfer proteins from large formate 2-D gel (18 cm) to membrane	Run gel, transfer proteins on a membrane, membrane stain with Coomassie, image capture with a flatbed scanner (no Western analysis)	$350 per blot
Western analysis (1-D gel, up to 10 wells) - using client supplied primary antibody	Run gel, transfer proteins to membrane, probe with antibodies, ECL detection and image capture with a Fuji LAS 3000 CCD camera	$300 per Western blot
Western analysis (1-D gel, up to 18 wells) - using client supplied primary antibody	Run gel, transfer proteins to membrane, probe with antibodies, ECL detection and image capture with a Fuji LAS 3000 CCD camera	$325 per Western blot
Western analysis (11 cm 2-D gel) - using client supplied primary antibody	Run gel, transfer proteins to membrane, probe with antibodies, ECL detection and image capture with a Fuji LAS 3000 CCD camera	$360 per Western blot
Western analysis (17 cm 2-D gel) - using client supplied primary antibody	Run gel, transfer proteins to membrane, probe with antibodies, ECL detection and image capture with a Fuji LAS 3000 CCD camera	$450 per Western blot
BioPlex/Luminex - instrument (daily access)	Instrument calibration and/or validation, and operation (Robot and plate reading not included)	$150 per day
BioPlex/Luminex - multiplex analysis DIY	Using an EpMotion robot for assay or plate reading with BioPlex/Luminex, etc.	$150 per hour
BioPlex/Luminex - multiplex analysis	Instrument calibration and/or validation, conducting assay using an EpMotion robot, plate reading and report writing	$800 per plate + kit cost
Instrument access DIY	Access to any instruments (limitations apply)	$150 hour