Amino Acid Analysis
Amino Acid Analysis is one of the best quantitative methods in protein chemistry and used by many laboratories for peptide/protein characterization. Whilst a well established technique in protein chemistry, complications can come from a variety of sources including
- variability in the inherent lability of amino acids to hydrolysis, oxidation and modification
- variability in instrument performance and hydrolysis/derivatisation conditions
- background or sample contamination
- data reduction methods
- work habits
APAF has refined Amino Acid Analysis methodologies ensuring accurate and reliable results from a broad range of sample types including
- food stuffs
- traditional medicines
- recombinant protein sources
- plant materials
The standard quantitative assay gives 16 amino acids, Histidine, Alanine, Isoleucine, Arginine, Leucine, Lysine, Aspartic Acid, Methionine, Phenylalanine, Glutamic acid, Threonine, Glycine, Valine, Proline, Serine, Tyrosine
Asparagine and Glutamine, are converted and measured in their acidic forms Aspartic and Glutamic Acid.
Cysteine and Tryptophan are each separate assays.
For further details or advice on this APAF service please contact us at aaa@proteome.org.au.