Mass Spectrometry Infrastructure
APAF has the state-of-the-art mass spectrometry infrastructure available for services and research, including:
AB SCIEX 4800Plus Proteomics Analyser - automated MALDI-TOF/TOF
Used for MALDI-TOF (PMF) and MALDI-TOF/TOF (PMF + MS/MS) protein identification services.
Waters Q-TOF Ultima API - LC ESI MS/MS
Coupled with Waters CapLC system and used for ESI analysis of intact protein masses and elemental composition analysis of organic compounds.
AB SCIEX QSTAR Elite - nanoLC ESI MS/MS
AB SCIEX QSTAR XL - nano-LC ESI MS/MS
Coupled with Eksigent 1D nanoLC and Agilent nanoLC systems. Used for 1D nanoLC ESI MS/MS and 2D nanoLC ESI MS/MS for protein identifications and biomarker discovery using iTRAQ technique.
AB SCIEX 5500 QTRAP – nanoLC and CapLC ESI MS/MS System
Coupled with Waters Acquity 2D nano UPLC, this instrument is used for targeted protein quantitative analysis.
AB SCIEX 4000 QTRAP – nanoLC ESI MS/MS System
Coupled with an Eksigent nanoLC-2D System and used for MRM biomarker screens and protein quantitation.
AB SCIEX Triple TOFTM 5600 System
Bruker Microflex – MALDI-TOF
Used primarily for protein intact mass determination.
Thermo LTQ – nanoLC ESI MS/MS
Coupled with Eksigent nanoLC system and used for nanoLC ESI MS/MS protein identification and label free protein quantitation.
Capillary LC systems
In addition, a number of capillary LC systems from Waters and Agilent are available for off-line sample clean up and fractionation.
All of these mass spectrometers and LC systems are operated by APAF scientists and some of them are available for use by trained clients under an access fee arrangement. Please contact
APAF if you have any queries.