N-Terminal Sequencing uses a chemical process based on the technique developed by Pehr Edman in the 1950's
N-terminal protein sequence information continues to play a significant role in modern structural and molecular biology. N-terminal sequencing (also called edman sequencing) is most commonly used to identify unknown proteins, confirm protein identity and quality (often for quality control of recombinant proteins), and identify protein N-terminus and cleavage sites. Long sequences of 50 amino acids or more are possible with this technique.
N-terminal sequencing utilises the well-established Edman degradative chemistry, sequentially removing amino acid residues from the N-terminus of the protein and identifying them by reversed phase HPLC. Pure proteins (>90%) usually generate easily interpreted data, but insufficiently purified protein mixtures may also provide useful data.
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Pricing for N-Terminal/Edman sequencing